|
KMID : 0606920030110020112
|
|
Biomolecules & Therapeutics
2003 Volume.11 No. 2 p.112 ~ p.118
|
|
|
Inhibition of Glutamate-Induced Change in Mitochondrial Membrane Permeability in PC12 cells by 1-Methylated β-carbolines
|
|
Han ES
Lee CS
|
|
|
Abstract
|
|
|
|
1-Methylated β-carbolines (harmaline and harmalol) and antioxidants (N-acetylcysteine and ascorbate) reduced the loss of cell viability in differentiated PC 12 cells treated with 5 mM glutamate. β-Carbolines prevented the glutamate-induced decrease in mitochondrial membrane potential, cytochrome c release and caspase-3 activation in PC 12 cells. β-Carbolines reduced the formation of reactive oxygen species and depletion of glutathione due to glutamate in PC12 cells. β-Carbolines revealed a scavenging action on hydrogen peroxide and reduced the iron and EDTA-mediated degradation of 2-deoxy-D-ribose. The results suggest that I-methylated β-carbolines attenuate the cytotoxic effect of glutamate on PC12 cells by reducing the alteration of mitochondrial membrane permeability that seems to be mediated by oxidative stress.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|